Genetically Engineered Mouse Models for Neuroinflammation

Inbred female C57BL/6J, Rag1^-/-, Rag2^-/-, IL-6^-/- and IL-10^-/- mice were obtained from the Jackson Laboratory (Bar Harbor, ME). IL-17A^-/-, CD11cdnTGF-RII, and CD4dnTGF-RII mice were described previously (38 (link)–40 (link)). IL-17RC^-/- mice were developed by Genentech and were obtained from the Mutant Mouse Regional Resource Center (University of California, Davis). IL-17RA^-/- and IL-17RA^-/-RC^-/- were developed in our Lab and described previously (41 (link)). IL-17RD^-/- mice were obtained from the Mutant Mouse Regional Resource Center (University of California, Davis). All mice used in this study have a B6 background and were bred in-house. Only female (6 to 8-wk-old) mice were used in the study as male mice are more resistance to HSV-IL-2-induced CNS demyelination than female mice (21 (link)). This is comparable to observations made in other models for MS.