Soil Chemistry and Enzyme Activities Measurement

Soil pH value (soil to water ratio of 1:2.5) was measured with a pH meter (FE28-Standard, METTLER-TOLEDO, Switzerland; Sun et al., 2022 (link)). SOC was determined by dichromate oxidation and titration with ferrous sulfate. TN was determined using the Kjeldahl digestion distillation method. Available nitrogen (AN) and phosphorus (AP) were measured using alkali hydrolyzation and Bray method, respectively (Akhtar et al., 2018 (link)). Soil enzyme activities of invertase (INV), urease (UE), acid phosphatase (ACP), catalase (CAT), PPO, and POD were determined using kits from Beijing Solarbio Technology Co. Ltd. according to the test instructions (Tabatabai, 1994 ; Saiya-Cork et al., 2002 (link)).

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Sun L., Zhang J., Zhao J., Lu X., Xiao C., Xiao Z., Zhang T., Gu Y., Sun H., Liu H, & Li Y. (2023). Effects of Cinnamomum camphora coppice planting on soil fertility, microbial community structure and enzyme activity in subtropical China. Frontiers in Microbiology, 14, 1104077.

Publication 2023

FE28-Standard

Acid phosphatase Alkali Catalase Digestion Distillation Enzyme activities Ferrous sulfate Invertase Nitrogen Phosphorus Titration Urease

Corresponding Organization : Yangtze University

Other organizations : Jiangxi Academy of Forestry

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Variable analysis

independent variables

Soil pH value
Soil organic carbon (SOC)
Total nitrogen (TN)
Available nitrogen (AN)
Available phosphorus (AP)
Invertase (INV) activity
Urease (UE) activity
Acid phosphatase (ACP) activity
Catalase (CAT) activity
Polyphenol oxidase (PPO) activity
Peroxidase (POD) activity

dependent variables

Soil pH value
Soil organic carbon (SOC)
Total nitrogen (TN)
Available nitrogen (AN)
Available phosphorus (AP)
Invertase (INV) activity
Urease (UE) activity
Acid phosphatase (ACP) activity
Catalase (CAT) activity
Polyphenol oxidase (PPO) activity
Peroxidase (POD) activity

control variables

Soil to water ratio (1:2.5)

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