Transcriptional Analysis of Fungal Genes

The transcriptional analyses for the genes were performed as reported previously [21 (link)]. The wild type strain was cultured on a SDAY plate, and the mycelia were sampled at the indicated time point. The total RNA was extracted from the mycelial samples with RNAiso^TM Plus Reagent (TaKaRa, Dalian, China) according to the manufacturer’s protocol. The cDNA was reverse transcribed using the PrimeScript^® RT reagent Kit (TaKaRa) and used as templates to perform the qRT-PCR reaction on a Mastercycler^® EP Realplex (Eppendorf, Hamburg, Germany) cycler. All primers are shown in Table S1. The relative expression level of each gene was calculated as the relative expression of different time points over 2 d using the 2^−∆∆CT method [22 (link)]. Fungal 18S rRNA was as an internal reference.