Quantifying Retinal Morphology and Vascularization

Morphometric analyses of the retinas were performed using Image J software and Adobe Photoshop software. To determine the amount of regression and neovascularization, the number of pixels in the avascular regression area and neovascular area was measured using the Lasso tool of Adobe Photoshop software as described 54 (link), and divided by the number of pixels in the total retinal area and presented as a percentage. RBC leakage was measured as TER119-stained area outside the vessels divided by total measured area of retina as previously described 70 (link). Macrophage infiltration was measured as the total number of F4/80⁺ macrophage in the whole retina.