Isolation and Characterization of Oleuropein Compound

OC was extracted from EVOO (The Governor, batch 5-214000-242017) [27 (link),28 (link),29 ] using liquid-liquid extraction process and further purification has been done using Sephadex LH-20 column purity of OC was determined using HPLC analysis on a Shimadzu high-performance liquid chromatography (HPLC) system equipped with ultra violet (UV)/Visible variable wavelength detector and a purity of >99% was confirmed [27 (link),28 (link),29 ]. Quantitative ¹H NMR in deutrated chloroform-d₃ (CDCl₃) was acquired on a JEOL Eclipse ECS-400 nuclear magnetic resonance (NMR) spectrometer. OC and LP dissolved in dimethyl sulfoxide (DMSO) to provide a 25 mM stock solution. These stock solutions used to prepare various treatment concentrations. The final concentration of DMSO maintained the same in all treatment groups within a given experiment and never exceeded 0.1 %.

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Siddique A.B., Ebrahim H.Y., Akl M.R., Ayoub N.M., Goda A.A., Mohyeldin M.M., Nagumalli S.K., Hananeh W.M., Liu Y.Y., Meyer S.A, & El Sayed K.A. (2019). (−)-Oleocanthal Combined with Lapatinib Treatment Synergized against HER-2 Positive Breast Cancer In Vitro and In Vivo. Nutrients, 11(2), 412.

Publication 2019

high-performance liquid chromatography (HPLC) system Eclipse ECS-400

Chloroform Dimethyl sulfoxide High performance liquid chromatography Liquid liquid extraction Nuclear magnetic resonance Sephadex lh 20 Violet

Corresponding Organization : University of Louisiana at Monroe

Other organizations : Jordan University of Science and Technology

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Variable analysis

independent variables

Concentration of oleuropein aglycone (OC)

dependent variables

Purity of OC

control variables

Dimethyl sulfoxide (DMSO) concentration, maintained at a maximum of 0.1% in all treatment groups

controls

Positive control: Not mentioned
Negative control: Not mentioned

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