High-field NMR protocol with automated setup

The spectrometer contained a Bruker AVANCE III HD (Bruker BioSpin GmbH, Rheinstetten, Germany) console combined with a 14.1-T magnet for 1H 600 MHz. It was equipped with a 5-mm inverse triple resonance CryoProbe (1H/13C/15N) with cold preamplifiers for 1H and 13C and a z-axis gradient with automated tuning and matching. The spectrometer contained a Bruker SampleJet system (Bruker Bipspin AG, Fällanden, Switzerland) set to 5-mm shuttle mode with a cooling rack for keeping samples at 279 K. The data were acquired and processed using Topspin 3.2 (Bruker BioSpin GmbH, Rheinstetten, Germany), and experiments were run under automation using the IconNMR program (Bruker BioSpin GmbH, Rheinstetten, Germany). The experiments were conducted using standard pulse sequences, namely noesygppr1d, cpmgpr1d, and zg30 (Topspin 3.2, Bruker Biospin GmbH, Rheinstetten, Germany) [19 (link)].

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Tsai C.K., Lin C.Y., Kang C.J., Liao C.T., Wang W.L., Chiang M.H., Yen T.C, & Lin G. (2020). Nuclear Magnetic Resonance Metabolomics Biomarkers for Identifying High Risk Patients with Extranodal Extension in Oral Squamous Cell Carcinoma. Journal of Clinical Medicine, 9(4), 951.

Publication 2020

AVANCE III HD Topspin 3.2 IconNMR program

Axis Cold Pulse Resonance

Corresponding Organization : Chang Gung Memorial Hospital

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Variable analysis

independent variables

Bruker AVANCE III HD console
14.1-T magnet for 1H 600 MHz
5-mm inverse triple resonance CryoProbe (1H/13C/15N) with cold preamplifiers for 1H and 13C and a z-axis gradient with automated tuning and matching
Bruker SampleJet system set to 5-mm shuttle mode with a cooling rack for keeping samples at 279 K

dependent variables

NMR data

control variables

Standard pulse sequences: noesygppr1d, cpmgpr1d, and zg30

controls

No positive or negative controls were explicitly mentioned.

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