Three hiPSC lines were used in this work. NESTIN-GFP knockin reporter (NES-GFP) and ND2-0 hiPSC were obtained from the NIH Center for Regenerative Medicine. hiPSC line USCK7 was generated in-house from human urine-derived cells by Cytotune (Life Technologies) reprogramming kit [21 (link)]. All hiPSCs were cultured on Matrigel-coated dishes in TeSR-E8 medium (Stemcell Technologies).
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