CITE-seq Analysis of Human PBMC

To further evaluate the validity of our method. We generated an in-house CITE-seq dataset of human PBMC from a healthy donor under IRB approval from the University of Pittsburgh. 1372 cells were stained with Totalseq-A from BioLegend and are prepared using the 10x Genomics platform with Gel Bead Kit V2. The prepared assay is subsequently sequenced on an Illumina Hiseq with a depth of 50K reads per cell. Cells in this dataset are measured for their surface marker abundance through CITE-seq (3 (link)). Ten surface markers are measured for every cell: CD3, CD4, CD8a, CD11c, CD14, CD16, CD19, CD56, CD127 and CD154. Cell Ranger 3.0 was used to process the data and generate UMI matrix for the downstream analysis.