Bacterial DNA Sequencing and Analysis

The PCR product with clear bands was sent to 1
^st Base Laboratories in Singapore for sequencing. The sequencing program was performed by ABI PRISM 3730×L GENETIC ANALYZER (Applied Biosystems, USA). The sequenced products were blasted (NCBI Basic Local Alignment Search Tool)
^{26 (link)}, and the results were presented as homology (%) of bacterial DNA sequences to the database sequences
²⁷.

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Nursyirwani N., Asmara W., Wahyuni A.E., Triyanto T., Fauzi M, & Muchlisin Z.A. (2017). Phenotype and genotype of lactic acid bacteria (LAB) isolated from the tiger grouper Epinephelus fuscoguttatus alimentary tract. F1000Research, 6, 1984.

Publication 2017

ABI PRISM 3730×L GENETIC ANALYZER

Bacterial dna Prism

Corresponding Organization :

Other organizations : Riau University, Universitas Gadjah Mada, Syiah Kuala University

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Variable analysis

independent variables

PCR product with clear bands

dependent variables

Homology (%) of bacterial DNA sequences to the database sequences

control variables

Not explicitly mentioned

controls

No positive or negative controls were mentioned in the provided information.

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