Cells were transduced using the ViraSafe Lentiviral Packaging System (Cell Biolabs). The dCas9–KRAB and SunTag–VP64 systems were used for CRISPRi and CRISPRa, respectively, as described previously61 (link). NQO1 was silenced both with short hairpin RNA (shRNA) and with CRISPRi constructs. NQO1-AS and HNRNPC were similarly silenced using CRISPRi. CTCF and HNRNPA2B1 were silenced using small interfering RNA (siRNA). NQO1 and NQO1-AS were overexpressed using CRISPRa. The shRNA and guide RNA sequences are shown in Supplementary Table 1 .
Free full text: Click here
