Bacterial Volatile Organic Compound Analysis

Bacteria were cultivated overnight in 6 ml liquid medium (170 rpm, 30°C) and subsequently transferred into the VOC collection system (Kai et al., 2010 (link)) containing 100 ml medium. Volatiles were collected and trapped on the adsorbent material Porapak (Sigma–Aldrich, Munich, Germany) and eluted in 24 h time intervals using dichloromethane (Carl Roth GmbH+Co., KG, Karlsruhe, Germany) and an internal standard (nonylacetate, final concentration 5 ng/μl). Finally, eluates were analyzed by gas chromatography/mass spectrometry according to the procedure published in Domik et al. (2016a) (link).

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Magnus N., Weise T, & Piechulla B. (2017). Carbon Catabolite Repression Regulates the Production of the Unique Volatile Sodorifen of Serratia plymuthica 4Rx13. Frontiers in Microbiology, 8, 2522.

Publication 2017

Porapak dichloromethane

Bacteria Dichloromethane Gas chromatography mass spectrometry

Corresponding Organization : University of Rostock

Other organizations : Euroimmun Medizinische Labordiagnostika (Germany)

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Variable analysis

independent variables

Cultivation of bacteria overnight in 6 ml liquid medium (170 rpm, 30°C)

dependent variables

Volatiles collected and trapped on the adsorbent material Porapak
Volatiles eluted in 24 h time intervals using dichloromethane and an internal standard (nonylacetate, final concentration 5 ng/μl)
Eluates analyzed by gas chromatography/mass spectrometry

control variables

Cultivation of bacteria in 100 ml medium in the VOC collection system (Kai et al., 2010)

controls

No positive or negative controls were explicitly mentioned.

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