Antibacterial Potency Screening of Compounds

AST was performed on the compounds (1–29) (Supplementary Table 1) (Yele et al., 2020 (link), 2021 ) concerning the standard protocol (CLSI, 2017 ). The lowest concentration of a compound at which inhibition of bacterial growth was observed is termed MIC. The microbroth dilution method in Hi-media, Mueller Hinton Broth (MHB), was used to calculate the MIC. Bacterial cultures were diluted at a ratio of 1:1,000 MHB to achieve turbidity of 0.5 McFarland. DMSO and ciprofloxacin were used as a negative and positive control, respectively. The bacterial inoculum was added into a series of 96-well plates containing various test compounds ranging from 0.625 to 20 μg/mL. Plates were incubated for 20–24 h at 37°C. The absorbance was recorded at 600 nm of wavelength using Tecan-i-control, 1.7.1.12. MIC determinations for each compound were carried out in triplicate using a duplicate sample each time (Eloff, 1998 (link); Agyare et al., 2012 (link)).