Conjugation of DSPE-PEG2000-NOTA was confirmed by matrix-assisted laser desorption ionization/time of flight (MALDI/TOF) mass spectroscopy. The purified product (1 mg) was added to an ultraflextreme (Bruker Daltonics, Billerica, MA, USA) mass spectrometer using sinapinic acid as a matrix (Bruker Daltonics) in Korea Basic Science Institute (KBSI, Daejeon, Republic of Korea).
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