Experiments were performed in vitro with strips of both circular (n = 12) and longitudinal (n = 13) muscles from human gastric fundus and their mechanical activities were recorded as changes in isometric force. These experiments were conducted using standard organ bath techniques, as previously described in another study by our laboratory [26 (link),27 (link)].
Electrical field stimulation (EFS) of intramural nerves was carried out by giving stimuli of various frequencies and durations (0.3 ms in trains of 1–20 Hz for 1–20 s, 150 V) which were applied via the two platinum ring electrodes attached to each strip. The electrodes were connected to a GRASS S88 (GRASS Instruments, Quincy, MA, USA) stimulator.
Electrical field stimulation (EFS) of intramural nerves was carried out by giving stimuli of various frequencies and durations (0.3 ms in trains of 1–20 Hz for 1–20 s, 150 V) which were applied via the two platinum ring electrodes attached to each strip. The electrodes were connected to a GRASS S88 (GRASS Instruments, Quincy, MA, USA) stimulator.
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