Canine Cytokine and Inflammatory Biomarkers

Blood samples were available on all study days from 9 of 10 dogs. One dog was excluded from blood sampling because of development of hospital‐associated anxiety upon repeated visits. Complete blood count, serum biochemistry, and C‐reactive protein (CRP) concentrations were performed using blood samples collected from recipients at the Central Laboratory of the VMTH. For cytokines, serum interleukins (IL)‐2, ‐6, ‐8, and tumor necrosis factor (TNF)‐α were measured using a multiplex electrochemiluminescence immunoassay and Canine Proinflammatory Panel 3 Ultrasensitive Kit (Meso Scale Discovery, Meso Scale Diagnostics, Rockville, Maryland) on a MSD Quickplex SQ 120 (Meso Scale Diagnostics, Rockville, Maryland), according to manufacturer instructions and as previously described.
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Lee M.A., Questa M., Wanakumjorn P., Kol A., McLaughlin B., Weimer B.C., Buono A., Suchodolski J.S, & Marsilio S. (2024). Safety profile and effects on the peripheral immune response of fecal microbiota transplantation in clinically healthy dogs. Journal of Veterinary Internal Medicine, 38(3), 1425-1436.

Publication 2024

Canine Proinflammatory Panel 3 Ultrasensitive Kit MSD Quickplex SQ 120

Corresponding Organization : University of California, Davis

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Complete blood count
Serum biochemistry
C-reactive protein (CRP) concentrations
Serum interleukins (IL)-2, -6, -8
Tumor necrosis factor (TNF)-α

control variables

Blood samples were available on all study days from 9 of 10 dogs
One dog was excluded from blood sampling because of development of hospital-associated anxiety upon repeated visits

positive controls

None mentioned

negative controls

None mentioned

Annotations

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