Enzymatic Grafting of Acrylic Acid on JRDF

Sodium hydroxide (6.25 mol∙L⁻¹, 24 mL), dH₂O (180 mL), and acrylic acid (15 mL) were mixed in a triangular flask and shaken in the EZC-004H oscillator at 65 r∙min⁻¹ and 25 °C [23 (link)]. Forty minutes later, 2 g of JRDF-UE was added, and suspension was continuously shaken at 65 r∙min⁻¹ for ten minutes. Next, thiosalicylic acid (58.4 mmol∙L⁻¹, 1.5 mL) and potassium thiosulfate (74 mmol∙L⁻¹, 2.25 mL) were added in sequence. The reaction mixture was shaken at 195 r∙min⁻¹ and 70 °C. Three hours later, the suspension was filtered through a nylon sieve with an aperture of 100 μm, and the residue was washed using anhydrous ethanol (30 mL) in triplicate. After heating at 50 °C for 8 h, JRDF modified via enzymolysis assisted by acrylate grafting (JRDF-EAG) was obtained. The grafting degree was determined by following the same procedures as Rani et al. [23 (link)].