Immunization of Mice with PE and SRBCs

For the phycoerythrin (PE) immunization the mice were injected subcutaneously with immunogen emulsion comprising 100 μl CFA (Thermo Fisher Scientific), 15 μg of R-PE (ProZyme, Cedarlane), and 100 μl PBS, vortexed for 45 min prior to the injection. For the immunization with sheep red blood cells (SRBCs), the mice were injected intravenously with 10⁹ SRBCs (Innovative Research IC10-0210, Cedarlane) in 300uls of PBS. Immunization protocols were previously described (24 (link), 61 (link)).

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Liang Y., Wang H., Seija N., Lin Y.H., Tung L.T., Di Noia J.M., Langlais D, & Nijnik A. (2024). B-cell intrinsic regulation of antibody mediated immunity by histone H2A deubiquitinase BAP1. Frontiers in Immunology, 15, 1353138.

Publication 2024

CFA

Corresponding Organization :

Other organizations : McGill University, McGill Genome Centre, Université de Montréal, Montreal Clinical Research Institute

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Variable analysis

independent variables

Injection route: subcutaneous for phycoerythrin (PE) immunization, intravenous for sheep red blood cells (SRBCs) immunization

dependent variables

Not explicitly mentioned

control variables

Volume of immunization: 100 μl for PE, 300 μl for SRBCs
Composition of immunization: 100 μl CFA, 15 μg of R-PE, 100 μl PBS for PE; 10^9 SRBCs in PBS for SRBCs
Vortexing time: 45 min prior to injection for PE immunization

controls

Positive control: None mentioned
Negative control: None mentioned

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