Latency Assay of CD34+ Hematopoietic Progenitor Cells

MRC-5 lung fibroblasts (ATCC), HEK293T/17 cells (ATCC), Sl/Sl stromal cells (Stem Cell Technology), M2-10B4 stromal cells (Stem Cell Technology), and CD34⁺ HPCs were maintained as previously described [17 (link)]. Human CD34⁺ HPCs were isolated from de-identified medical waste following bone marrow isolations from healthy donors for clinical procedures at the Banner-University Medical Center at the University of Arizona. Latency assays were performed as previously described [15 (link), 17 (link)].

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Buehler J., Carpenter E., Zeltzer S., Igarashi S., Rak M., Mikell I., Nelson J.A, & Goodrum F. (2019). Host signaling and EGR1 transcriptional control of human cytomegalovirus replication and latency. PLoS Pathogens, 15(11), e1008037.

Publication 2019

MRC-5 lung fibroblasts HEK293T/17 cells Sl/Sl stromal cells M2-10B4 stromal cells

Assays Bone marrow Cells Donors Fibroblasts Hpcs Human Isolations Lung Medical waste Stem cell Stromal cells

Corresponding Organization : Oregon Health & Science University

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Variable analysis

independent variables

Cell lines: MRC-5 lung fibroblasts, HEK293T/17 cells, Sl/Sl stromal cells, M2-10B4 stromal cells, and CD34+ HPCs

dependent variables

Latency assays

control variables

Cell lines were maintained as previously described [17 (link)]

controls

Positive and negative controls are not explicitly mentioned in the provided information.

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