C. elegans expression clones were made in a derivative of pPD49.26 (A. Fire), the pSM vector (a kind gift from S. McCarroll and C. I. Bargmann). dsh-1 cDNA clone was obtained by RT-PCR from N2 mRNA using Superscript III First-strand synthesis system and Phusion High-Fidelity DNA Polymerase (Thermo Fisher Scientific). lin-17 cDNA clone was obtained from the plasmid used in the previous work (Mizumoto and Shen, 2013 (link)).
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