C. elegans Expression Clone Generation
Corresponding Organization : University of British Columbia
Variable analysis
- C. elegans expression clones made in a derivative of pPD49.26 (A. Fire), the pSM vector (a kind gift from S. McCarroll and C. I. Bargmann)
- Dsh-1 cDNA clone obtained by RT-PCR from N2 mRNA using Superscript III First-strand synthesis system and Phusion High-Fidelity DNA Polymerase (Thermo Fisher Scientific)
- Lin-17 cDNA clone obtained from the plasmid used in the previous work (Mizumoto and Shen, 2013)
- Not explicitly mentioned
- Not explicitly mentioned
- Positive control: Not mentioned
- Negative control: Not mentioned
Annotations
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