GABA Receptor Dynamics in Alfaxalone Sedation

Large outward GABA_A‐R currents were initiated using the same methodology as stated prior. GABA_A‐R current decay time, integrated area under the curve, and peak amplitude were determined using the same methodology as stated prior. Decay time, integrated area under the curve, peak amplitude, and baseline holding current were then normalized to whole‐cell capacitance. The protocol was repeated with telencephalon slices obtained from alfaxalone sedated goldfish at 2, 3, and 4–6 h following alfaxalone application. The timepoints were binned so that any measurement performed between 1.5 and 2.5 h was considered 2 h following whole‐animal alfaxalone sedation, any measurement performed between 2.5 and 3.5 h was considered 3 h following whole‐animal alfaxalone sedation, and any measurement performed between 3.5 and 6.5 h was considered 4–6 h following whole‐animal alfaxalone sedation. Measurements made on the 30‐min mark were placed in the later time group. Following slice preparation, occurring 1.5 h after alfaxalone sedation, slices were washed every 30 min, with the aCSF housing the tissue slices being replaced with fresh alfaxalone‐free aCSF.