Cells were grown in 12 and 96 well plates and treated with dexamethasone, compound 4e at the concentration 10 nM and 0.1 µM for 24 h. Osteoblasts cells were washed twice with PBS and then incubated with 5 μM 2′,7′-dichlorofluorescein diacetate (DCFH-DA) for 45 min at 37 °C. Fluorescent images were captured using Evos Fl Fluorescent microscope (Applied Biosystems) at × 10 magnification and absorbance was measured by Spectra max M2 Molecular Devices at 485/535 nm. [35 (link)].