We purchased mannosidase II rabbit polyclonal antibodies (Affinity Bioreagents, Golden, CO), Rab43 mouse monoclonal antibodies (Abnova, Taipei City, Taiwan), Rab43 rabbit polyclonal antibodies (Atlas Antibodies, Stockholm, Sweden), VAMP4 rabbit polyclonal antibodies (Sigma-Aldrich, St. Louis, MO), and protein disulfide isomerase mouse monoclonal antibodies (ThermoFisher, Waltham, MA). V5 antibodies and the various Alexa Fluor–conjugated secondary antibodies were obtained from Invitrogen (Waltham, MA), and secondary antibodies and actin mouse monoclonal antibodies were obtained from LI-COR. Endoglycosidase H was purchased from New England Biolabs (Ipswich, MA). The polyclonal VSV (Mire et al., 2010 (link)), AE1 (Adair-Kirk et al., 1999 (link)), and I1 anti-G monoclonal antibodies (Lefrancois and Lyles, 1982 (link)) have been described. COS7, AD293, and PH5CH8 cells (Ikeda et al., 1998 (link)) were grown in DMEM containing 10% fetal calf serum.
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Antibody Reagents and Cell Lines
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Actin
Anti antibodies
Antibodies
Cells
Endoglycosidase
Fetal calf serum
Mannosidase ii
Monoclonal antibodies
Mouse
Protein disulfide isomerase
Rabbit
Corresponding Organization : University of Oxford
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Variable analysis
independent variables
- Mannosidase II rabbit polyclonal antibodies
- Rab43 mouse monoclonal antibodies
- Rab43 rabbit polyclonal antibodies
- VAMP4 rabbit polyclonal antibodies
- Protein disulfide isomerase mouse monoclonal antibodies
- V5 antibodies
- Alexa Fluor–conjugated secondary antibodies
- Secondary antibodies
- Actin mouse monoclonal antibodies
- Endoglycosidase H
- Not explicitly mentioned
- COS7 cells
- AD293 cells
- PH5CH8 cells
- DMEM containing 10% fetal calf serum
- Polyclonal VSV (Mire et al., 2010)
- AE1 (Adair-Kirk et al., 1999)
- I1 anti-G monoclonal antibodies (Lefrancois and Lyles, 1982)
- Not explicitly mentioned
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