Recombinant Human MIF and Antagonist Preparation

Recombinant human MIF (rhMIF) was prepared as described elsewhere [24 (link)] (endotoxin content < 0.1 EU/mL). MIF antagonist MIF098 [3-(3-hydroxybenzyl)-5-methylbenzooxazol-2-one] was dissolved in DMSO at a concentration of 149 µM [25 (link)]. The neutralizing anti-MIF monoclonal antibody (clone NIHlllD.9) was obtained from ascites after purification using protein A/G spin column and resuspended at 5.15 mg/mL [26 (link),27 (link)]. The cytokine neutralizing antibodies anti-IL-6 and anti-IL-1β (BioLegend, San Diego, CA, USA) were obtained.

Free full text: Click here

Trifone C., Baquero L., Czernikier A., Benencio P., Leng L., Laufer N., Quiroga M.F., Bucala R., Ghiglione Y, & Turk G. (2022). Macrophage Migration Inhibitory Factor (MIF) Promotes Increased Proportions of the Highly Permissive Th17-like Cell Profile during HIV Infection. Viruses, 14(10), 2218.

Publication 2022

anti-IL-6 anti-IL-1β

Ascites Clone Cytokine Dmso Endotoxin Human Il 1β Mif098 Neutralizing antibodies Protein g

Corresponding Organization : Consejo Nacional de Investigaciones Científicas y Técnicas

Other organizations : Fundación Ciencias Exactas y Naturales, Yale University

Top 5 similar protocols

Variable analysis

independent variables

Recombinant human MIF (rhMIF)
MIF antagonist MIF098 [3-(3-hydroxybenzyl)-5-methylbenzooxazol-2-one]
Neutralizing anti-MIF monoclonal antibody (clone NIHlllD.9)
Anti-IL-6 and anti-IL-1β cytokine neutralizing antibodies

dependent variables

Not explicitly mentioned

control variables

Endotoxin content < 0.1 EU/mL in recombinant human MIF (rhMIF)

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!