Human bladder TCC cell lines h-UM-UC-3, h-T24, and h-5637 were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA). Cell lines were authenticated via short tandem repeat DNA profiling by Genetica DNA Laboratories (Burlington, NC, USA). h-T24 cells were grown in McCoy’s media (GE Healthcare Bio-Sciences, Pittsburgh, PA, USA), h-UM-UC-3 cells were grown in minimum essential media (GE Healthcare Bio-Sciences), and h-5637 cells were grown in RPMI-1640 media (GE Healthcare Bio-Sciences) supplemented with 10% fetal bovine serum, 100 IU penicillin, and 100 μg/mL streptomycin. Canine TCC (K9TCC) cell lines, K9TCC#1Lillie and K9TCC#5Lilly, were established and characterized in the laboratory of Dr Cekanova as described previously in detail.40 (link),48 ,49 (link) K9TCC#1Lillie and K9TCC#5Lilly were grown in complete RPMI-1640 media supplemented with 10% fetal bovine serum, 100 IU penicillin, and 100 μg/mL streptomycin. Cells were grown at 37°C and 5% CO2.
Protocol detail
Establishment and Characterization of Bladder and Canine Transitional Cell Carcinoma Cell Lines
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Bladder
Canine
Cell lines
Cells
Fetal bovine serum
Human
Penicillin
Short tandem repeat
Streptomycin
Corresponding Organization : University of Tennessee at Knoxville
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Variable analysis
independent variables
- Cell lines (h-UM-UC-3, h-T24, h-5637, K9TCC#1Lillie, K9TCC#5Lilly)
- Not explicitly mentioned
- Culture media (McCoy's media, minimum essential media, RPMI-1640 media)
- Supplementation (10% fetal bovine serum, 100 IU penicillin, 100 μg/mL streptomycin)
- Temperature (37°C)
- CO2 concentration (5%)
- Positive control: Not mentioned
- Negative control: Not mentioned
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