EdU Staining for Cell Proliferation

EdU staining was conducted using the BeyoClick™ EdU Cell Proliferation Kit with Alexa Fluor 594 (Beyotime, Cat. No: C00788L). Cells were washed with PBS. Fresh DMEM was added, and then, 10 μM EdU was added into the medium. The cells were incubated for 2 hours at 37°C/5% CO₂. After the incubation, the cells were washed with PBS to remove the DMEM and the free EdU probe [36 (link)]. The cells were then fixed in 4% paraformaldehyde at room temperature for 30 minutes before being stained with DAPI for 3 minutes. After an additional wash in PBS, the cells were observed under an inverted microscope [37 (link)].

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Zhao S., Li P., Wang P., Yang J., Song P., Zhang D, & Zhou G. (2019). Nurr1 Promotes Lung Cancer Apoptosis Via Enhancing Mitochondrial Stress and p53-Drp1 Pathway. Open Life Sciences, 14, 262-274.

Publication 2019

BeyoClick™ EdU Cell Proliferation Kit with Alexa Fluor 594

Alexa 594 Cell proliferation Cells Dapi Microscope Paraformaldehyde

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Variable analysis

independent variables

EdU concentration (10 μM)

dependent variables

Cell proliferation (EdU staining)
Nuclear staining (DAPI)

control variables

Cell culture medium (DMEM)
Cell incubation conditions (37°C, 5% CO2, 2 hours)

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