8-triazaspiro(4.5)decan-4-one,8-(3-(p-fluorobenzoyl)propyl)-1-phenyl-3 hydrochloride (spiperone), 1H-Benzo(6,7)cyclohepta(1,2,3-de)pyrido(2,1-a)isoquinolin-3-ol, 2,3,4,4a,8,9,13b,14-octahydro-3-(1,1-dimethylethyl)-(3S-(3-alpha,4a-alpha,13b-beta)) ((+)-butaclamol) hydrochloride and S-(−)-3-chloro-5-ethyl-N-[(1-ethyl-2-pyrrolidinyl)methyl]-6-hydroxy-2-methoxybenzamide hydrochloride (eticlopride), were purchased from Sigma-Aldrich. The synthesis and purification of the SV series of compounds, including 1-((5-methoxy-1H-indol-3-yl)methyl)-4-(4-(methylthio)phenyl)piperidin-4-ol (SV 293), have been previously reported (Vangveravong et al., 2006 (link); Vangveravong et al., 2011). The synthesis and purification of the 4-(dimethylamino)-N-(4-(4-(2-methoxyphenyl)piperazin-1-yl)butyl)benzamide (WC-10) and N-[(4-[4-[2-(2-fluoroethoxy)phenyl]-1-piperazinyl]butyl]-4-(3-thienyl)benzamide (LS-3-134) have been previously reported (Tu et al., 2011a (link)).
LS-3-134 was radiolabeled with tritium ([3H]LS-3-134) by American Radiolabeled Chemicals, Inc. (St. Louis, MO) to a specific activity of 60 Ci/mmol. [125I]IABN was prepared and purified as previously described (Luedtke et al., 2000 (link)). [3H]raclopride was obtained commercially (NEN-PerkinElmer).
LS-3-134 was radiolabeled with tritium ([3H]LS-3-134) by American Radiolabeled Chemicals, Inc. (St. Louis, MO) to a specific activity of 60 Ci/mmol. [125I]IABN was prepared and purified as previously described (Luedtke et al., 2000 (link)). [3H]raclopride was obtained commercially (NEN-PerkinElmer).
