Immunohistochemical labeling of embryonic brain sections or dissociated neural cells was performed as described previously [65 (link),66 (link)]. The following primary antibodies were used: Chicken anti-GFP (Invitrogen), Rabbit anti-GFP (Invitrogen), mouse anti-β-III Tubulin (Phosphosolutions), rabbit anti-acetyl-α-tubulin (Cell Signaling), and mouse anti-β-tubulin (Upstate). Appropriate secondary antibodies conjugated with Alexa Fluor dyes (Invitrogen) were used to detect primary antibodies. Polymerized F-actins were detected by labeling with phalloidin-Alexa 568 (Invitrogen).
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Immunohistochemical Labeling of Neural Cells
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Alexa 568
Antibodies
Brain
Chicken
Dyes
Embryonic
Mouse
Neural cells
Phalloidin
Polymerized actins
Rabbit
Tubulin
α tubulin
Corresponding Organization :
Other organizations : Nebraska Medical Center, University of Nebraska Medical Center
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independent variables
- Immunohistochemical labeling of embryonic brain sections or dissociated neural cells
- Localization and expression of proteins detected by the primary antibodies: Chicken anti-GFP, Rabbit anti-GFP, mouse anti-β-III Tubulin, rabbit anti-acetyl-α-tubulin, and mouse anti-β-tubulin
- Localization of polymerized F-actins detected by phalloidin-Alexa 568
- Procedures for immunohistochemical labeling as described previously in references [65] and [66]
- Not explicitly mentioned
- Not explicitly mentioned
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