Tissues were washed with 0.1× PBS several times at room temperature with gentle shaking, and incubated with the primary antibodies in 6% (w/v) bovine serum albumin (BSA), 0.2% (v/v) Triton X-100, 0.01% (w/v) sodium azide in 0.1× PBS for 1~2 days in a 37℃ shaker. Antibodies against Calbindin (1:500, AB1778, Millipore), Collagen type IV (1:500, AB769, Millipore), DCX (1:500, SC-8066, Santa Cruz), GFAP (1:500, z0334, DAKO), GFP (1:1000, ab13970, Abcam), Iba1 (1:500, 019-19741, WACO), Laminin (1:500, L9393, Sigma) and TH (1:1000, AB152, Millipore) were used. Samples were washed several times with 0.1× PBS and incubated with secondary antibodies (1:500) for 1~2 days in a 37℃ shaker that matched the host of each primary antibody for fluorescence imaging. Samples were incubated in CUBIC-mouse solution [9 (link)] for at least 1 h. Images were acquired using a TCS SP8 confocal laser-scanning microscope (Leica, Germany). Image reconstructions were performed using LAS X software (Leica) or Imaris imaging software (Bitplane). After imaging, samples were stored in PBS at 4℃.