Thirty days post-infection, mice received 2 × 106 bone marrow-derived M2 macrophages by the intra-tracheal route or were treated with recombinant IL-33 (BioLegend, San Diego, CA) (1 μg/mouse) by the intranasal route (five doses total at 2-day intervals) as previously reported51 (link)62 (link). A CFU assay was performed at 8 days post-adoptive cell transfer or 24 hours after the last treatment with IL-33.
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