Fluorescently tagged fusion constructs of PDLP5 (UniProt Q8GUJ2) or its cytoplasmic tail mutants were produced using overlapping PCR by Phusion high‐fidelity DNA polymerase (New England Biolabs), and purified DNA fragments were subsequently cloned into the Gateway entry vector pENTR/D‐TOPO (ThermoFisher Scientific) and destination vector pGWB. To create pBI‐D vector expression cassettes, sequences from the pBI121 binary vector were replaced by expression cassettes containing a Cauliflower mosaic virus (CaMV) 35 S promoter with dual enhancers, a 5′ nontranslated leader sequence from Tobacco etch virus (Carrington and Freed, 1990 (link)), and a CaMV 35 S terminator. The PDLP5 3C‐3A mutant was cloned into the binary vector pBI‐D, introduced into the Agrobacterium tumefaciens strain GV3101 by electroporation, and used to create transgenic N. benthamiana lines. Optimized Yellow fever virus envelope protein (YFE‐1) (UniProt P03314) and B. anthracis protective antigen (PA83) (UniProt P13423) genes were cloned into a pGreen‐based expression vector carrying TMV genome sequences from pBID4 (Musiychuk et al., 2007 ). The resulting constructs were introduced into the A. tumefaciens strain AGL1 by electroporation. All constructs were confirmed by Sanger sequencing before agro transformation. Additional information regarding plasmids and vectors used in the current study is provided in Table S1.