Evaluating Skin Barrier Function in Atopic Dermatitis

AD severity was evaluate clinically using SCORing Atopic Dermatitis (SCORAD) (31 (link)). Skin barrier integrity was quantified using transepidermal water loss (TEWL) as measured on lesional skin using the DermaLab TEWL probe (Cortex Technology, Hadsund, Denmark). Skin barrier dysfunction was quantified by expression of filaggrin (FLG) from lesional keratinocytes normalized to the expression of the 18S rRNA, as described previously (2 (link), 32 (link)).

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Gill K., Moore C., Nwogu O., Kroner J.W., Chang W.C., Stevens M.L., kyzy A.B., Biagini J.M., Devonshire A.L., Kottyan L., Schwartz J.T., Assa’ad A.H., Martin L.J., Andorf S., Hershey G.K, & Roskin K.M. (2023). B cell repertoire in children with skin barrier dysfunction supports altered IgE maturation associated with allergic food sensitization. bioRxiv.

Publication Preprint 2023

18s rrna Atopic dermatitis Cortex Filaggrin Keratinocytes Skin

Corresponding Organization :

Other organizations : Cincinnati Children's Hospital Medical Center, University of Cincinnati Medical Center

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

AD severity evaluated clinically using SCORAD (SCORing Atopic Dermatitis)
Skin barrier integrity quantified using transepidermal water loss (TEWL) on lesional skin
Skin barrier dysfunction quantified by expression of filaggrin (FLG) from lesional keratinocytes normalized to the expression of the 18S rRNA

control variables

None explicitly mentioned

controls

No positive or negative controls were explicitly mentioned in the provided information.

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