PEO1 cells were maintained in RPMI+ 10% heat inactivated FBS (Avantor, Cat. #10803-034) + 100 μg/mL Penicillin/Streptomycin (Lonza, Cat. #09-757F). EHMT1 or EHMT2 overexpression cells were obtained by using lentivirus to infect cells with either TRE_EHMT1_hygromycin or TRE_EHMT2_hygromycin. To produce lentivirus, 500,000 Lenti-X 293T cells were seeded on 6-well plate. Twenty-four hours later, the cells were transfected with packaging plasmids (0.5μg pCMV-VSV-G, 1.1μg pD8.9), 0.85μg transfer plasmid, 7.35μg of PEI MAX (Polysciences Inc., Cat. #24765-1) and 10mM HEPES. Media was changed 24 hours later. Supernatant with virus was collected 48 and 72 hours post transfection. The supernatant was filtered using a 0.45μm PES Filter membrane (Whatman Uniflo, Cat. #9914-2504). Cells were then infected using 1mL of supernatant with 5μg/mL polybrene (EMD Millipore, Cat. #TR-1003-G). Media was changed 24 hours later. 48 hours post transduction, cells were selected using 200μg/ml Hygromycin B (Biosciences, Cat. #31282-04-9). Cells were selected until death of non-transduced cells. Once cells were selected, they were induced for EHMT1 or EHMT2 expression using Doxycycline (1μg/ml, TCI, Cat. #D4116) for four days and collected. Cells were immunoblotted for EHMT1 (Bethyl Laboratories, Cat. #A301-642A; dilution 1:500), EHMT2 (Cell Signaling, Cat. #3306; RRID:AB_2097647; dilution 1:1000), alpha-tubulin (Cell Signaling Cat. #3873; RRID:AB_1904178; dilution 1:3000), and H3K9me2 (Cell Signaling Cat. #4658; RRID:AB_10544405; diluted 1:1000).