Behavioral Analysis of AD Mouse Models

Wild-type (WT) C57BL/6 mice (male, 25–30 g) were procured from the Shanghai Laboratory Animal Center (Shanghai, China). KCa3.1^−/− mice were obtained from the Jackson Laboratory (Bar Harbor, ME, USA) and housed as described previously [18 (link)]. APP/PS1 transgenic mice (an AD model) were also purchased from the Jackson Laboratory (no. 003378) [15 (link)]. KCa3.1^−/− mice were crossed with APP/PS1 mice and the offspring were intercrossed to generate mice with the KCa3.1^−/−/APP/PS1 genotype. Mouse cohorts of the four genotypes (WT, KCa3.1^−/−, KCa3.1^−/−/APP/PS1, and APP/PS1) were generated and used for behavioral analysis. The protocols of all animal experiments were approved by the Animal Experimentation Ethics Committee of Shanghai Jiao Tong University School of Medicine, Shanghai, China (ethics protocol number: A-2015-010).