Protocol detail

Optical and Spectroscopic Characterization

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UV–VIS analysis was carried out using an Agilent 8453 diode array spectrophotometer [44 (link)]. Fluorescence study was carried out using a Jasco FP-750 spectrofluorometer (JASCO, Tokio, Japan). Quantum yield was determined by the comparative method described in our previous article [44 (link)]. The study PL-pH dependence was carried out at pH = 4, 5, 6, 7, 8, 9. The pH was adjusted using an Elmetron CX-551 pH-meter (combined glass pH electrode, Elmetron, Zabrze, Poland). The solvatochromism study was carried out using the following solvents: water, acetone, acetonitrile, tetrahydrofuran, and methanol. Infrared analysis was performed using a Nexus 470 diamond crystal ATR FTIR spectrophotometer (Thermo Fisher Scientific (Waltham, MA, USA).

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Janus Ł., Radwan-Pragłowska J., Piątkowski M, & Bogdał D. (2020). Smart, Tunable CQDs with Antioxidant Properties for Biomedical Applications—Ecofriendly Synthesis and Characterization. Molecules, 25(3), 736.

Publication 2020

8453 diode array spectrophotometer FP-750 spectrofluorometer CX-551 pH-meter

Acetone Acetonitrile Diamond Fluorescence Ftir Methanol Nexus Solvents Tetrahydrofuran

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Variable analysis

independent variables

PH (4, 5, 6, 7, 8, 9)
Solvents (water, acetone, acetonitrile, tetrahydrofuran, methanol)

dependent variables

UV-VIS absorption
Fluorescence emission
Quantum yield

control variables

Agilent 8453 diode array spectrophotometer for UV-VIS analysis
Jasco FP-750 spectrofluorometer for fluorescence study
Comparative method for quantum yield determination
Elmetron CX-551 pH-meter for pH adjustment
Nexus 470 diamond crystal ATR FTIR spectrophotometer for infrared analysis

controls

Positive control: Not mentioned
Negative control: Not mentioned

Annotations

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