Liver samples were accurately weighed and homogenized (diluted 1:10) in Phosphate buffered saline (PBS) (Solarbio, Beijing, China) with a tissue crusher. The homogenization was 900 × g centrifuge for 10 min at 4°C and retained the supernatant. The protein content of the homogenate was measured using Folin-phenol reagent (16 (link), 17 (link)). The contents of albumin (ALB), cholesterol (CHO), high-density lipoprotein cholesterol (HDLC), low-density lipoprotein cholesterol (LDLC), glucose (GLUC), creatine kinase (CK), iron (IRON), magnesium (MG), calcium (CA), phosphate (PHOS), alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine aminotransferase (ALT) and creatinine (CREA) were detected by Roche Cobas C311 automatic biochemical analyzer (Shanghai, China). The kits used were Cobas C311 products.
According to the product instructions, amylase (AMS), glucose-6-phosphate dehydrogenase (G-6-PD), malic enzyme (ME), pyruvate kinase (PK), acyl CoA oxidase (ACO), lipase (LPS), fatty acid synthase (FAS), carnitine acyl carnitine translocation (CACT), acetyl CoA carboxylase (ACC), lipoprotein lipase (LPL), superoxide dismutase (SOD), Phospholipid hydroperoxide glutathione peroxidase (GSH-PX), catalase (CAT) and malondialdehyde (MDA) were detected. See Table S1 for product numbers.
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