His-tagged fibronectin (domains 7–10) was expressed from pET15bFN-III7-10RGE [19 (link)] in E.coli BL21(DE3)pLysS (Promega, cat. no. L1195) and purified using Ni-NTA chromatography. Aliquots of the purified protein were frozen in liquid nitrogen and stored in PBS (pH 7.4) at a concentration of 1 mg ml−1 at −80 °C.
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