Specific pathogen-free C57BL/6, B6(Cg)-Tyrc-2J/J, and IL-10gfp reporter transgenic mice on a C57BL/6 background were acquired from The Jackson Laboratory (B6.129S6-Il10tm1Flv/J stock # 8379, “TIGER”, and B6(Cg)-Il10tm1.1Karp/J stock #14530, “Vert-X) and bred in house with B6(Cg)-Tyrc-2J/J (albino B6) for heterozygosity and homozygosity. 6–12-wk-old adult mice were used in all experiments. B6(Cg)-Tyrc-2J/J (albino B6) were also used where indicated. All mice were housed under specific pathogen–free conditions (including MNV, MPV, and MHV) and maintained on standard rodent chow and water supplied ad libitum.
For adoptive T cell transfer, spleens and nodes were removed from OT-I dsRed mice (as described in [26 (link), 60 (link)]). Cells were purified to > 90% purity using an Automacs (Miltenyi Biotech) and using negative selection for CD8+ T cells. At least one day prior to infection, 1 x 105 cells were transferred into IL-10gfp animals.
For adoptive T cell transfer, spleens and nodes were removed from OT-I dsRed mice (as described in [26 (link), 60 (link)]). Cells were purified to > 90% purity using an Automacs (Miltenyi Biotech) and using negative selection for CD8+ T cells. At least one day prior to infection, 1 x 105 cells were transferred into IL-10gfp animals.
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