Culturing and Serum Opsonization of F. alocis

F. alocis ATCC 38596 was cultured in brain heart infusion (BHI) broth supplemented 5 mg/mL yeast extract, L-cysteine (0.05%), and arginine (0.05%) for 7 days anaerobically at 37°C as previously described (20 (link), 37 (link)). Serum opsonization was performed by incubating F. alocis at 37°C for 20 min in 10% normal human serum (Complement Technology, Inc., Tyler, TX, USA). Heat-killed F. alocis was generated by incubation at 90°C for 60 min. Non-viability was confirmed by incubation in culture media at the same conditions used for the live organism.

Free full text: Click here

Miralda I., Vashishta A., Rogers M.N., Rouchka E.C., Li X., Waigel S., Lamont R.J, & Uriarte S.M. (2020). Whole Transcriptome Analysis Reveals That Filifactor alocis Modulates TNFα-Stimulated MAPK Activation in Human Neutrophils. Frontiers in Immunology, 11, 497.

Publication 2020

normal human serum

Arginine Brain Conditions culture media Heart Human L cysteine Opsonization Serum Yeast

Corresponding Organization : University of Louisville

Top 5 similar protocols

Variable analysis

independent variables

Serum opsonization: Incubating F. alocis in 10% normal human serum at 37°C for 20 min
Heat-killed F. alocis: Incubation at 90°C for 60 min

dependent variables

Bacterial viability: Confirmed by incubation in culture media at the same conditions used for the live organism

control variables

Culture conditions: Brain heart infusion (BHI) broth supplemented with 5 mg/mL yeast extract, L-cysteine (0.05%), and arginine (0.05%), incubated anaerobically at 37°C for 7 days

controls

Positive control: Live F. alocis
Negative control: Heat-killed F. alocis

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!