Mass Spectrometry Analysis of Elutions

Elutions were analyzed using an Applied Biosystems API 3000 triple quadrupole mass spectrometer with electrospray ionization (Foster City, CA) as previously described (Tortoriello et al., 2013 (link), Leishman et al., 2016b ). 20 µL from each elution were chromatographed using XDB-C18 reversed phase HPLC analytical column (Agilent) and optimized mobile phase gradients. Mobile phase A: 20% methanol, 80% water (v/v) and 1 mM ammonium acetate (Sigma Aldrich). Mobile phase B: 100% methanol, 1 mM ammonium acetate. Two Shimadzu 10ADvp pumps (Columbia, MD) provided the pressure for gradient elution. Every method run began with 0% mobile phase B, reached a state of 100% mobile phase B flowing at 0.2 mL per minute, and gradually returned to 0% mobile phase B.

Free full text: Click here

Leishman E., Kunkler P.E., Manchanda M., Sangani K., Stuart J.M., Oxford G.S., Hurley J.H, & Bradshaw H.B. (2017). Environmental toxin acrolein alters levels of endogenous lipids, including TRP agonists: A potential mechanism for headache driven by TRPA1 activation. Neurobiology of Pain, 1, 28-36.

Publication 2017

XDB-C18 reversed phase HPLC analytical column ammonium acetate 10ADvp pumps

Ammonium acetate Hplc Methanol Pressure

Corresponding Organization :

Other organizations : Indiana University Bloomington, Indiana University – Purdue University Indianapolis, Neurosciences Institute

Top 5 similar protocols

Variable analysis

independent variables

Mobile phase gradient

dependent variables

Elutions analyzed using mass spectrometry

control variables

Sample volume (20 µL)
Chromatography column (XDB-C18 reversed phase HPLC analytical column)
Mobile phase A (20% methanol, 80% water (v/v) and 1 mM ammonium acetate)
Mobile phase B (100% methanol, 1 mM ammonium acetate)
Flow rate (0.2 mL per minute)
Mass spectrometer (Applied Biosystems API 3000 triple quadrupole mass spectrometer with electrospray ionization)

controls

Positive control not explicitly mentioned
Negative control not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!