Plasmid Constructs and RNA Interference for lnc-AROD and CUEDC2

lnc-AROD, CUEDC2, and lnc-AROD-ORF1/ORF2-FLAG were cloned into the pCDH-CMV-MCS-EF1-copGFP plasmid (P0268; MiaolingBio, Wuhan, China); these constructs were named pCDH-AROD, pCDH-ORF1, pCDH-ORF2, and pCDH-CUEDC2. The full-length lnc-AROD, lnc-AROD-del, CUEDC2–3′-UTR, and CUEDC2–3′-UTR-del were inserted into the pGLO plasmid (Promega, Madison, WI, USA) and were named pGLO-AROD, pGLO-AROD-DEL, pGLO-CUEDC2–3′-UTR, and pGLO-CUEDC2–3′-UTR-del. Argonaute2 (AGO2) was cloned into the PCDH-3flag-EGFP plasmid (catalog number 21538; Addgene), and lnc-AROD was cloned into the pMS2 plasmid, which was gifted from Myriam Gorospe (47 (link)). lnc-AROD-specific siRNAs and CUEDC2-specific siRNAs were designed by Thermo RNAi Designer and transfected into human lung adenocarcinoma epithelial (A549) cells using jetPRIME transfection reagent (Polyplus, France) according to the manufacturer's instructions. The siRNA sequences used in this study are listed in Table S1. The miRNA mimics or inhibitors were synthesized by GenePharma (Shanghai, China), and their sequences are listed in Table S1.