An Illumina TruSeq PCR-free DNA library with 500 bp insert size of an affected Rough Collie (CL013) was prepared. We collected 227 million 2 × 150 bp paired-end reads on a NovaSeq 6000 instrument (26.9× coverage). Mapping and alignment were performed as described [12 (link)]. The sequence data were deposited under study accession PRJEB16012 and sample accession SAMEA4867926 at the European Nucleotide Archive.
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