Generation and analysis of mouse models

C57BL/6 (B6) and B6.SJL mice were purchased from the National Cancer Institute. Tak1^fl/fl, Tak1^fl/flCd4^Cre, Tbx21^GFP (T-bet^GFP), Nr4a1^GFP (Nur77^GFP), Bcl2l11^–/– (Bim^–/–), Rag2^GFP, Klf2^GFP and B2m^–/– mice were described previously^{8 (link),9 (link),24 (link),40 (link),41 (link)}. Ifng^YFP, I-Ab^–/–, Cd1^–/–, Cd4^CreERT2, Cd28^–/– and Rosa26^TdT (Jax 007914) were obtained from Jackson Laboratories. Il15^–/– and K^bD^b–/– mice were obtained from Taconic. Rag2^–/– mice were obtained from Jackson Laboratories and bred with B6.SJL mice to establish a CD45.1/2⁺Rag2^–/– line. For chimeras, lethally irradiated recipients were reconstituted with T cell depleted donor bone marrow cells and provided with neomycin- and proximicin B supplemented water for two weeks. Chimeras were analyzed minimum eight weeks after reconstitution. All animal experiments were approved by the Institutional Animal Care and Use Committee of the University at Minnesota.