Some infected IL-10 KO and WT animals were injected with 2 × 106 CTV+ CD4 T cells 3.5 h before sacrifice (i.p.) and 40 μg of DyLight488 labeled Lycopersicon esculentum (tomato) Lectin (catalog no. DL-1174, Vector Laboratories, Burlingame, CA) 20 min before sacrifice (i.v.). CellTrace Violet (catalog no. C34557, Thermo Fisher Scientific, Waltham, MA) labeling was performed as previously described [55 (link)].
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