Rat adrenal pheochromocytoma (PC12) cells were purchased from American Type Culture Collection (ATCC, cat. no. CRL-1721). CPCGI was obtained from Jilin Buchang Pharmaceutical Co., Ltd. DMEM high-sugar medium, horse serum, and fetal bovine serum were purchased from Gibco (Thermo Fisher Scientific, Inc.).
PC12 cells were cultured in DMEM containing 10% fetal bovine serum and 5% horse serum, and cultured at 37°C with 95% humidity and 5% CO2 (20 (link)). PC12 cells were divided into control, model, model + vehicle (PBS) and model + CPCGI. In the model + vehicle and model + CPCGI groups, PC12 cells were pre-treated with PBS and CPCGI separately for 1 h before stimulation with 50 µM Aβ25–35 (Sigma-Aldrich; Merck KGaA) for 24 h. PC12 cells in the model group were stimulate with 50 µM Aβ25–35 for 24 h (21 (link)). Cells in the control group were not subjected to any treatment. Subsequently, PC12 cells in each group were subjected to the following experiments.
PC12 cells were cultured in DMEM containing 10% fetal bovine serum and 5% horse serum, and cultured at 37°C with 95% humidity and 5% CO2 (20 (link)). PC12 cells were divided into control, model, model + vehicle (PBS) and model + CPCGI. In the model + vehicle and model + CPCGI groups, PC12 cells were pre-treated with PBS and CPCGI separately for 1 h before stimulation with 50 µM Aβ25–35 (Sigma-Aldrich; Merck KGaA) for 24 h. PC12 cells in the model group were stimulate with 50 µM Aβ25–35 for 24 h (21 (link)). Cells in the control group were not subjected to any treatment. Subsequently, PC12 cells in each group were subjected to the following experiments.
