Cartilage constructs were fabricated as previously described13 (link). Briefly, F344 rat ASCs were suspended in type I oligomeric collagen (4.0 mg/ml) and polymerized. They were then compressed to 0.5-mm-thick constructs (75 mg/ml) and cultured in chondrogenic differentiation medium (Hyclone Advance Stem Chondrogenic Differentiation Medium, SH30889.02; Thermo Scientific, Waltham,MA) and were maintained in a 37oC, 5% CO2 incubator for up to 4 weeks. The medium was changed twice per week.
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Fabrication of Cartilage Constructs
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Other organizations : Purdue University West Lafayette
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Variable analysis
independent variables
- Fabrication of cartilage constructs
- Compression of constructs to 0.5-mm-thick
- Culture duration (up to 4 weeks)
- Chondrogenic differentiation of F344 rat ASCs
- Cell type (F344 rat ASCs)
- Type I oligomeric collagen concentration (4.0 mg/ml)
- Construct density (75 mg/ml)
- Culture conditions (37°C, 5% CO2 incubator)
- Culture medium (Hyclone Advance Stem Chondrogenic Differentiation Medium)
- Medium change frequency (twice per week)
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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