Poly (caprolactam) (nylon 6, 181110), formic acid (FA, F0507), thrombin from bovine plasma (T4648), fibrinogen from bovine plasma (F8630), streptozotocin (STZ, S0130) and gelatin from porcine skin (G1890) were purchased from Sigma-Aldrich Co. (St. Louis, MO). 1,1,1,3,3,3-Hexafluoro-2-propanol (HFIP, 003409) was purchased from Oakwood Products, Inc. (Estill, SC). Calcium chloride (CaCl2, BDH9224) and sodium chloride (NaCl, BDH9286) were purchased from VWR International (Radnor, PA). Barium chloride dihydrate (BaCl2.2H2O, BX0060-1) was purchased from EMD Millipore Corporation. Regular sodium alginate (PROTANAL LF 10/60FT) and sterile sodium alginate (Pronova SLG 100) were purchased from FMC BioPolymer Co. (Philadelphia, PA). Sucrose (8360-6) was purchased from Avantor Performance Materials, LLC. (Center Valley, PA). All reagents were used without further purification. Sulfobetaine-modified alginate (SB-alginate) was synthesized according to a previously published protocol[18 (link)].
Protocol detail
Nanofiber Scaffold Fabrication and Characterization
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Alginate
Bacl2
Barium chloride dihydrate
Biopolymer
Bovine
Calcium chloride
Caprolactam
Fibrinogen
Formic acid
Gelatin
Hexafluoro 2 propanol
Nacl
Nylon 6
Plasma
Poly
Porcine
Skin
Sodium alginate
Sterile
Sucrose
Sulfobetaine
Thrombin
Corresponding Organization : Cornell University
Other organizations : Donghua University, Boston Children's Hospital, Harvard University, Novo Nordisk (Denmark), Harvard Stem Cell Institute
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Variable analysis
independent variables
- Poly (caprolactam) (nylon 6, 181110)
- Formic acid (FA, F0507)
- Thrombin from bovine plasma (T4648)
- Fibrinogen from bovine plasma (F8630)
- Streptozotocin (STZ, S0130)
- Gelatin from porcine skin (G1890)
- 1,1,1,3,3,3-Hexafluoro-2-propanol (HFIP, 003409)
- Calcium chloride (CaCl2, BDH9224)
- Sodium chloride (NaCl, BDH9286)
- Barium chloride dihydrate (BaCl2.2H2O, BX0060-1)
- Regular sodium alginate (PROTANAL LF 10/60FT)
- Sterile sodium alginate (Pronova SLG 100)
- Sucrose (8360-6)
- Sulfobetaine-modified alginate (SB-alginate)
- Not explicitly mentioned
- All reagents were used without further purification
- Not specified
- Not specified
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