Lentiviral Knockdown of BARD1 Gene
Corresponding Organization : Ceinge Biotecnologie Avanzate (Italy)
Other organizations : Istituti di Ricovero e Cura a Carattere Scientifico, SDN Istituto di Ricerca Diagnostica e Nucleare
Variable analysis
- ShRNA targeting human BARD1 gene expression
- Two different shRNAs against FL BARD1: V2LHS_93186 and V3LHS_365581
- Non-silencing pGIPZ Lentiviral shRNAmir as control (RHS4346)
- BARD1 gene expression knockdown
- HEK293 cell line
- Transfection conditions (10µg of shRNA plasmid DNA, 30µl of Trans-Lentiviral packaging Mix, 25µl TrasFectin in 10mm plate)
- Harvesting and filtering of supernatants
- Transduction conditions and determination of lentivector Titre
- Puromycin selection for 100% GFP positive cells
- Non-silencing pGIPZ Lentiviral shRNAmir (RHS4346)
- Not explicitly mentioned
Annotations
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