Proteins were extracted from adherent cells and separated as previously described18 (link). Primary antibodies were as following: MGMT clone MT3.1 (#NB 100–692 Novus, MO) and anti-actin (clone C4, MAB1501 Millipore). Peroxydase-conjugated secondary antibodies (Jackson Immunoresearch Laboratories, PA) were detected using the ECL detection system (Amersham Biosciences, NJ) (N = 3).
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