Protein Extraction and Immunoblotting
Corresponding Organization :
Other organizations : Inserm, Université d'Angers, Nantes Université, University of Castilla-La Mancha, Sorbonne Université, Laboratoire Jacques-Louis Lions, Université de Strasbourg, Institut de génétique et de biologie moléculaire et cellulaire, Centre National de la Recherche Scientifique, Institut de Cancérologie de l'Ouest
Variable analysis
- None explicitly mentioned
- Protein expression levels of MGMT and actin
- Number of adherent cells from which proteins were extracted
- Protein extraction and separation method as previously described in ref. 18
- Primary antibodies used: MGMT clone MT3.1 and anti-actin (clone C4)
- Peroxydase-conjugated secondary antibodies detected using the ECL detection system
- Positive control: Not specified
- Negative control: Not specified
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