Immortalized Human Fimbrial Cell Lines

Three immortalized human fimbrial epithelial cell lines (FT282-V, FT282-cyclin E1 (CCNE1), FE25), one subline derived from the xenograft tumor of FE25 cells (FEXT2), and two genomically-proven HGSC cell lines (OVSAHO and KURAMOCHI) were used in this study. The relevant genotypes and phenotypes are shown in Table 1. We established FE25 cells by transduction using human papillomavirus (HPV) E6/E7 plus human telomerase reverse transcriptase (hTERT) [30 (link)]. FT282-CCNE1 and FT282-V cell lines, a kind gift from Dr. Ronny Drapkin, were transduced with TP53 p.R175H, hTERT plus CCNE1 or vector, respectively [23 (link)]. These cells were maintained in MCDB105/M199 medium (1:1, Merck, NJ, USA) supplemented with 10% fetal bovine serum (FBS, Thermo Fisher Scientific, Waltham, MA, USA) and penicillin/streptomycin (P/S, Corning Inc., Corning, NY, USA). The HGSC cell lines, KURAMOCHI and OVSAHO were obtained from the JCRB cell bank, Japan. The cells were cultured in RPMI 1640 medium (Gibco-Thermo Fisher Scientific, MA, USA) supplemented with 10% (v/v) FBS, penicillin (50 U/mL) and streptomycin (50 μg/mL).