The following cell lines were obtained from the National Institute of General Medical Sciences Human Genetic Cell Repository at the Coriell Institute for Medical Research: GM08399 (control) and GM18453 (I1061T/I1061T). All fibroblasts were grown in Advanced MEM (Gibco, 12492-013) plus 10% FBS (R&D Systems, S11150) plus PSG (Gibco, 10378016). WT, I1061T-NPC1, and null-NPC1 human Hap1 cells were previously described (38 (link)) and cultured in IMDM (Gibco, 12440061) plus 10% FBS.
Corresponding Organization : University of Michigan–Ann Arbor
Other organizations :
Hospital for Sick Children, University of Toronto, Eunice Kennedy Shriver National Institute of Child Health and Human Development, University of Illinois at Chicago
Cell type: WT, I1061T-NPC1, null-NPC1 human Hap1 cells
dependent variables
Not explicitly mentioned
control variables
Culture media: Advanced MEM (Gibco, 12492-013) plus 10% FBS (R&D Systems, S11150) plus PSG (Gibco, 10378016) for fibroblasts
Culture media: IMDM (Gibco, 12440061) plus 10% FBS for Hap1 cells
controls
Positive control: GM08399 (control) fibroblasts
Negative control: Not explicitly mentioned
Annotations
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